Secondary embryogenesis and plant regeneration in Psidium guajava L. (41)

Authors

  • Jorge A. Vilchez. P. Dpto. Botánica, Facultad de Agronomía, La Uniuersidad del Zulia, AP 15205, Maracaibo, Edo. Zulia. (4005ZU), Republica Bolivariana de Venezuela
  • Nilca R. Albany V. Dpto. Quimica, Facultad de Agronomía, La Universidad del Zulia, AP 15205, Maracaibo, Edo. Zulia. (4005ZU), Republica Bolivariana de Venezuela
  • Rafael Gomez Kosky Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas (UCLV), Carretera a Camajuni Km 5 1/2 Santa Clara, Villa Clara, Cuba CP 54830
  • Leyanis Garcia Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas (UCLV), Carretera a Camajuni Km 5 1/2 Santa Clara, Villa Clara, Cuba CP 54830

Keywords:

Myrtaceae, Guava, Somatic embryogenesis, In vitro culture, 2, 4-D

Abstract

With the objective of evaluating secondary somatic embryogenesis (SSE) and the germination of somatic embryos of guava (Psidium guajava L.) 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzylaminopurine (6-BAP) were tested, respectively. Fresh weight (FW) and number of secondary somatic embryos formed (NSSEF) in the SSE, and the germination percentage (GP) were determined. The greatest value of NSSEF (48.5; P < 0.05) was obtained with 1 mg L-1 of 2,4-D, eight weeks after culture initiation. The highest GP (83%; P < 0.05) was reached with 0.25 mg L-1 of 6-BAP by culturing the SE in groups. These results suggested that somatic embryogenesis may be a possible alternative for the rapid propagation of promising guava tree selections.

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Issue

Volume 81 Number 1

Section

Research Notes