ISSN: 2223-2133 (Online), 1815-8986 (Print)
Vol. 8 Number 2, December 2008
Seroprevalence of Leptospira in Dogs in Grenada, West Indies
Diana Stone, Ravindra Sharma, Harold Russell, Omowale Amukeru-Marshall, Marquite Gittens-St.Hilaire, Jill Caldwell, Emily Cappon
Leptospira bacteria infect numerous species of animals and cause serious disease in both dogs and humans. Leptospirosis is caused by many different serovars and canine vaccine protection is serovar-specific. The objetive of thisstudy was to determine the seroprevalence and serovars of Leptosira in dogs on the island of Grenada, West Indies. Serum samples from 105 dogs and were evaluated by the microscopic agglutination test (MAT) and by an Enzyme-Linked Immunosorbent Assay (ELISA) method adapted for canine Immunoglobulin M (IgM). Sera from 20 dogs were positive by MAT (19.0%) and 67 were positive by ELISA (64.0%). All MAT-positive sera were also ELISA-positive. Percent seropositive did not differ significantly by age, sex or health score of dog or among the 6 parishes of Grenada. Fourteen of the MET-positive samples had antibodies to multiple Leptospira serovars. All but one of the identified serovars were from the Icterohaemorrhagiae serogroup and included in decreading order of frequency copenhagen, mankarso and icterohaemorrhagiae RGA. One dog was identified with antibodies to serogroup Pyrogenes serovar pyrogenes. Identification of Leptospira serovars responsible for canine exposure in Grenada will facilitate appropriate recommendations for canine vaccination on the island.