ISSN: 2223-2133 (Online), 1815-8986 (Print)
Serological and sub clinical evidence of Johne's disease (Mycobacterium paratuberculosis) in water buffalo (Bubalus bubalis) on two farms in Trinidad
Karla Georges, C Ezeokoli, N Seepersadsingh, N Lambie, L Rastogi, J Ryan, M Diptee, A Adesiyun
Serum samples from 147 semi - intensively and 128 extensively reared water buffalo
(Bubalus bubalis) were tested using a commercial enzyme- linked immunosorbent assay
(ELISA) for Mycobacterium paratuberculosis. Whole blood samples from 125 of the
semi- intensively reared animals and 140 of the extensively reared animals were
stimulated using johnin purified protein derivative (PPD) and examined for gammainterferon (IFN-y) production.
Overall, 13.1% of water buffalo were serologically positive for M. paratuberculosis using
the ELISA and 13.2 % were positive tested using the IFN-y test. There was a significant
association between age and a sero- positive test result, (p= 0.007, chi square 1 df, 95%
confidence). This is the first report of serological and subclinical detection of M.
paratububerculosis in water buffalo in Trinidad. Further investigation is needed in order
to isolate the organism and develop a suitable strategy to cope with this disease of
worldwide economic importance.

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