ISSN: 0041-3216 (Online), 0041-3216 (Print)
Volume 65 Number 2
A simple technique to obtain nodulation of chickpea and some large-seeded legumes in agar tubes. (121)
A simple technique adapted to trap numerous air bubbles in agar deeps during their preparation was found to help nodulation of chickpea, soybean and other large-seeded legumes. Nodulation was consistently high in tubes of various sizes. Nodulation of chickpea and of soybean occurred at 2-4% agar and 0.75-2% agar concentration, respectively. The technique enhanced the precision of plant infection tests for enumerating chickpea rhizobia in soils. Nitrogen fixing (C2H2) activities of nodulated soybean plants could also be determined.
Keywords: Agar-tube technique; Chickpea; Large-seeded legumes; Enumeration of rhizobia; Soybean