ISSN: 0041-3216 (Online), 0041-3216 (Print)
Volume 81 Number 1
Secondary embryogenesis and plant regeneration in Psidium guajava L. (41)
With the objective of evaluating secondary somatic embryogenesis (SSE) and the germination of somatic embryos of guava (Psidium guajava L.) 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzylaminopurine (6-BAP) were tested, respectively. Fresh weight (FW) and number of secondary somatic embryos formed (NSSEF) in the SSE, and the germination percentage (GP) were determined. The greatest value of NSSEF (48.5; P < 0.05) was obtained with 1 mg L-1 of 2,4-D, eight weeks after culture initiation. The highest GP (83%; P < 0.05) was reached with 0.25 mg L-1 of 6-BAP by culturing the SE in groups. These results suggested that somatic embryogenesis may be a possible alternative for the rapid propagation of promising guava tree selections.
Keywords: Myrtaceae; Guava; Somatic embryogenesis; In vitro culture; 2,4-D