Secondary embryogenesis and plant regeneration in Psidium guajava L 41

Vilchez. P., Jorge A.; Albany V., Nilca R.; Kosky, Rafael Gomez; Garcia, Leyanis

ISSN: 0041-3216

ISSN: 0041-3216 (Online), 0041-3216 (Print)

Volume 81 Number 1

Research Notes

Secondary embryogenesis and plant regeneration in Psidium guajava L. (41)

Jorge A. Vilchez. P., Nilca R. Albany V., Rafael Gomez Kosky and Leyanis Garcia

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With the objective of evaluating secondary somatic embryogenesis (SSE) and the germination of somatic embryos of guava (Psidium guajava L.) 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzylaminopurine (6-BAP) were tested, respectively. Fresh weight (FW) and number of secondary somatic embryos formed (NSSEF) in the SSE, and the germination percentage (GP) were determined. The greatest value of NSSEF (48.5; P < 0.05) was obtained with 1 mg L-1 of 2,4-D, eight weeks after culture initiation. The highest GP (83%; P < 0.05) was reached with 0.25 mg L-1 of 6-BAP by culturing the SE in groups. These results suggested that somatic embryogenesis may be a possible alternative for the rapid propagation of promising guava tree selections.

Keywords: Myrtaceae; Guava; Somatic embryogenesis; In vitro culture; 2,4-D