The effect of 1-methylcyclopropene (1-MCP) concentrations (0, 500, 1000 and 2000 nL L-1) for 12 h on shelf life of three durian fruit cultivars (Arancillo. Duyaya, and Puyat) under ambient conditions was studied including various treatment durations (0, 6, 12 h with 1000 nL L-1 1-MCP). Commercially mature and uniform quality fruit were harvested, dipped in 500 ppm thiabendazole, air-dried, and treated with 1-MCP (Smart FreshTM) in a sealed airtight chamber and stored in ambient (28±2 ºC and 83.5 RH ±5%). Total soluble solids did not vary but weight loss at ripe stage of fruit treated with various 1 MCP concentrations differed and ranged from 5.1 to 14.1%. Visual quality retention was more pronounced in Puyat while Arancillo exhibited a rapid decline. 1000 nL L-11-MCP improved the shelf life of the three durian cultivars up to four days (a mean improvement of 45.4%) relative to the untreated control. Arancillo and Duyaya responded best with 6 hours treatment time while Puyat had significant reduction in ion leakage, delay in days to capsule splitting, and days to disease occurrence when applied with 1000 nL L-1 1-MCP at 12 hours. It also delayed ripening of fruit for up to three days.