Using molecular markers to determine the genetic relationships of medicinal plants

Abstract

In developed countries, the use of medicinal plants is currently drawing much attention, as essential constituents of complementary and alternative medications. In order to remove uncertainties regarding their safety and effectiveness, these plants need to be accurately identified and validated. In this regard, genetics has come to the fore as a dependable and quick method for the discerning of medicinal plant sources. This work was a DNA barcoding study, involving genetic and sequence investigates, in addition to a pair of ITS markers, conducted from five families for a total of nine plant varieties. The aim was to reveal the way in which, the four identified DNA barcoding areas of markers (ITS), facilitated the identification and conservation process, and to scrutinise their efficiency in DNA barcoding. The results showed that four sequences of DNA, for the plants under study, as Carissa macrocarpa, Alcea rosea, Delonix regia, and Roystonea regia. Therefore, it can be assumed that using DNA molecular is definitive, when it comes to discerning the genetic links in medicinal plants. From assessing population configurations and genetic diversity to phylogenetic research, facilitating reproduction programmes, and quality control activities, the application of genetic analysis is both wide-ranging and effective. The assessment of DNA markers, a protocol with conventional expertise, will serve to improve our information and knowledge of medicinal plants, while promoting their sustainable usage.