Comparison of different parts of lemon (Citrus limon) for DNA extraction

Authors

  • Mehrnoush Aminisarteshnizi Aquaculture Research Unit, School of Agricultural and Environmental Sciences, University of Limpopo (Turfloop Campus), South Africa

Keywords:

DNA extraction, Citrus limon, spectrophotometer, pcr

Abstract

The Citrus limon, popularly known as the lemon tree, is native to Asia. This species is cultivated commercially worldwide in tropical, semitropical, and warm temperate countries, including the Mediterranean region. Lemon cultivation and production play an essential role in the economy of many countries in which genomic DNA is critical for genetic diversity and molecular analysis of this tree. In this study, we compared three different parts of the lemon tree to extract DNA. The DNA from the lemon tree was extracted using the Chelex method from the fresh leaves, stems, and fruit. For this comparison, in 2021 three samples of C. limon from South Africa were used. The quantity and purity of DNA were measured using a spectrophotometer. To confirm and evaluate the extracted DNA, PCR amplification with 28S primers was used. The spectrophotometer results showed the highest quantity of nucleic acid extracted was from stem (63.18 ± 2 mg/ml). The ratio of 260/280 for quality of DNA extraction in the stem (1.8 ± 0.01) was in the acceptable range. The quantity of protein in the fruit (5.5 ± 1.2 mg/ml) was high. The amplification of the samples showed that the stem and leaf had high purity and concentration of DNA, but the stem gave the best results regarding high-quality DNA.

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Published

2022-11-29

Issue

Section

Research Notes