The characterization of cultivars of cowpeas, Vigna unguiculata (L.) Walp. And Vigna vexillata Benth. By electrophoresis of seed protection

S. Oghiakhe, R.J. Cooke, L.E.N. Jackai

Abstract


The possibility of using electrophoresis to characterize cultivars of cowpeas, Vigna unguiculata Walp. and Vigna vexillata Benth., was investigated. Sodium dodecyl sulphate - polyacrylamide electrophoresis (SDS-PAGE) did not reveal any useful genetic differences in total seed protein patl between cowpea cultivars. However, PAGE of the water-soluble seed proteins provided a useful analysis technique. A key for the classification of 15 cultivars into five groups based on the presence absence of three protein bands following PAGE of the water-soluble seed proteins was developed

Keywords


Cowpea (Vigna unguiculata); Vigna vexillata; Electrophoresis; Sodium dodecyl sulphc polyacrylamide gel electrophoresis (SDS--PAGE); Polyacrylamide gel electrophoresis (PAGE)

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