, Research Notes

The characterization of cultivars of cowpeas, Vigna unguiculata (L.) Walp. And Vigna vexillata Benth. By electrophoresis of seed protection

Research Notes
S. Oghiakhe
Department of Biochemistry and Biological Science, Wye College, University of London, Ashford, Kent, TN25 5AH, U.K.
R.J. Cooke
National Institute of Agricultural Botany, Huntingdon Road, Cambridge, England
L.E.N. Jackai
International Institute of Tropical Agriculture (IITA), PMB 5320, Oyo Road, Ibdan, Nigeria, West Africa
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Keywords

Cowpea (Vigna unguiculata)
Vigna vexillata
Electrophoresis
Sodium dodecyl sulphc polyacrylamide gel electrophoresis (SDS--PAGE)
Polyacrylamide gel electrophoresis (PAGE)

How to Cite

The characterization of cultivars of cowpeas, Vigna unguiculata (L.) Walp. And Vigna vexillata Benth. By electrophoresis of seed protection. (1993). Tropical Agriculture, 70(2). https://journals.sta.uwi.edu/ojs/index.php/ta/article/view/7092
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Abstract

The possibility of using electrophoresis to characterize cultivars of cowpeas, Vigna unguiculata Walp. and Vigna vexillata Benth., was investigated. Sodium dodecyl sulphate - polyacrylamide electrophoresis (SDS-PAGE) did not reveal any useful genetic differences in total seed protein patl between cowpea cultivars. However, PAGE of the water-soluble seed proteins provided a useful analysis technique. A key for the classification of 15 cultivars into five groups based on the presence absence of three protein bands following PAGE of the water-soluble seed proteins was developed
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