Biocontrol potential of a Burkholderia sp against tomato soil borne fungal pathogens
Keywords:Burholderia sp, biocontrol, tomato root pathogens, defense enzymes
AbstractThe biocontrol potential of a new Burkholderia sp strain-V-1 was evaluated under laboratory and greenhouse conditions. Tomato seeds, when treated with the bacterial suspension, showed significant increase in germination percentage, root and shoot length and seedling vigour. The Burkholderia sp strain-V-1 inhibited the mycelial growth of Pythium aphanidematum, Fusarium oxysporum f. sp solani, Rhizoctonia solani and Sclerotium rolfsii under in vitro conditions. A talc-based powder formulation of Burkholderia sp strain-1 was developed and evaluated for its efficacy in the management of tomato soil-borne pathogens under greenhouse conditions. Seed treatment combined with soil application of the talc powder formulation of Burkholderia sp significantly reduced the incidence of damping off, wilt, root rot and Southern stem blight in green house conditions. Bacterial inoculation in plants led to augmentation of phenolic levels and upregulation of activities of defense enzymes including phenyl alanine ammonia lyase (PAL), peroxidase (PO), polyphenol oxidase (PPO), ? 1,3 glucanase and chitinase in leaves and roots of tomato. The increase in phenolic levels and enhanced defense enzyme activities in roots were observed up to 7 days after inoculation.