, Research Papers

A simple technique to obtain nodulation of chickpea and some large-seeded legumes in agar tubes. (121)

Research Papers
K. Lakshminarayana
Department of Microbiology, Haryana Agricultural University, Hisar-125004, India
Narula Narula
Department of Microbiology, Haryana Agricultural University, Hisar-125004, India
P. Tauro
Department of Microbiology, Haryana Agricultural University, Hisar-125004, India
PDF

Keywords

Agar-tube technique
Chickpea
Large-seeded legumes
Enumeration of rhizobia
Soybean

How to Cite

A simple technique to obtain nodulation of chickpea and some large-seeded legumes in agar tubes. (121). (1988). Tropical Agriculture, 65(2). https://journals.sta.uwi.edu/ojs/index.php/ta/article/view/5390
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver AMA

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX

Abstract

A simple technique adapted to trap numerous air bubbles in agar deeps during their preparation was found to help nodulation of chickpea, soybean and other large-seeded legumes. Nodulation was consistently high in tubes of various sizes. Nodulation of chickpea and of soybean occurred at 2-4% agar and 0.75-2% agar concentration, respectively. The technique enhanced the precision of plant infection tests for enumerating chickpea rhizobia in soils. Nitrogen fixing (C2H2) activities of nodulated soybean plants could also be determined.
PDF