Meristem-tip culture of cocoa

Authors

  • Y. Adu-Ampomah Plant Breeding Unit, Joint FAO/IAEA Programme, Agency's Laboratories, International Atomic Energy Agency, PO Box 100, A-1400 Vienna Austria
  • F.J. Novak Plant Breeding Unit, Joint FAO/IAEA Programme, Agency's Laboratories, International Atomic Energy Agency, PO Box 100, A-1400 Vienna Austria
  • R. Afza Plant Breeding Unit, Joint FAO/IAEA Programme, Agency's Laboratories, International Atomic Energy Agency, PO Box 100, A-1400 Vienna Austria
  • M. van Duren Plant Breeding Unit, Joint FAO/IAEA Programme, Agency's Laboratories, International Atomic Energy Agency, PO Box 100, A-1400 Vienna Austria

Keywords:

Shoot tip, Tissue culture, Growth regulators

Abstract

Meristem tips were excised from terminal and axillary buds of young cocoa plants (2-4 weeks old) and cultured on media with 1 ?M zeatin and various concentrations of NAA and GA3. The subculturing of shoots in liquid medium was beneficial for nodal elongation and shoot growth. Gamma ray irradiation at low doses (1-4 Gy) helped to reduce callus formation. The roots were induced on half-strength MS medium with 10 ?M IBA, 0.1 ?M IAA and with reduced sucrose content (15 000 mg I-1)

Issue

Section

Research Papers