, Research Papers

Meristem-tip culture of cocoa

Research Papers
Y. Adu-Ampomah
Plant Breeding Unit, Joint FAO/IAEA Programme, Agency's Laboratories, International Atomic Energy Agency, PO Box 100, A-1400 Vienna Austria
F.J. Novak
Plant Breeding Unit, Joint FAO/IAEA Programme, Agency's Laboratories, International Atomic Energy Agency, PO Box 100, A-1400 Vienna Austria
R. Afza
Plant Breeding Unit, Joint FAO/IAEA Programme, Agency's Laboratories, International Atomic Energy Agency, PO Box 100, A-1400 Vienna Austria
M. van Duren
Plant Breeding Unit, Joint FAO/IAEA Programme, Agency's Laboratories, International Atomic Energy Agency, PO Box 100, A-1400 Vienna Austria
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Keywords

Shoot tip
Tissue culture
Growth regulators

How to Cite

Meristem-tip culture of cocoa. (1992). Tropical Agriculture, 69(3). https://journals.sta.uwi.edu/ojs/index.php/ta/article/view/4832
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Abstract

Meristem tips were excised from terminal and axillary buds of young cocoa plants (2-4 weeks old) and cultured on media with 1 ?M zeatin and various concentrations of NAA and GA3. The subculturing of shoots in liquid medium was beneficial for nodal elongation and shoot growth. Gamma ray irradiation at low doses (1-4 Gy) helped to reduce callus formation. The roots were induced on half-strength MS medium with 10 ?M IBA, 0.1 ?M IAA and with reduced sucrose content (15 000 mg I-1)
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