Abstract
Cold storage at 0.56 and 1.11°C showed probit 9 (99.9968%) mortality of third stage larvae of field-collected Mexican fruit fly, A. ludens (Loew), from a glass surface after 12 and 14 d, respectively. Probit 9 mortality of native populations of eggs and larvae of the same insect from mango, Mangifera indicum L., was determined after 13 to 16 d at 1.11 to 12.2 °C. In mango, at 0.56°C, West Indian fruit fly, A. obliqua (Loew), needed 5 to 7 fewer d for probit 9 mortality than that determined of A. ludens from mango. In grapefruit, Citrus grandis Macfadden, 1.11, 2.8, 6.7, 8.3 and 12.2°C caused probit 9 mortality of A. ludens pupae after 11 to 22 d. As the temperatures decreased no linear trend was observed for fewer days for probit 9 mortality. Cold-storage of grapefruit could be developed at 8.3 to 12.2°C as a quarantine treatment against A. ludens but 4 to 11 more d for probit 9 were required than at 1.11 to 6.7 °C. There was no difference in d to probit 9 mortalities in grapefruit between 1.11, 2.8 and 6.7 °C.