In vitro regeneration from immature cotyledon explant and protein profile changes during organogenesis in groundnut (Arachis hypogaea L.)

P. Venkatachalam, N. Geetha, N. Jayabalan

Abstract


Complete plants were regenerated from in vitro cultured immature cotyledon segments of groundnut (Arachis hypogaea L. cv. TMV-7) by organogenesis. Callus cultures were best initiated from immature cotyledon segments on MS (Murash1ge and Skoog) salts containing B5 vitamins supplemented with indole-3-acetic acid (IAA) and ?-naphthalene acetic acid (NAA; 4.0 mg L·l) and kinetin (KIN; 0.5 mg L ·1). Calluses were transferred to a medium containing KIN (2.0 mg L·1) and IAA and NAA (0. 5 mg L·1) for shoot initiation. The regenerated shoots were transferred to a medium containing indole-3-butyrlc acid (IBA; 2.0 mg L· 1) and KIN (0.2 mg L·1) for developing roots. In vitro produced plantlets developed successfully, matured, and set seed. The protein profiles (sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE)) of callus, callus with shoot, and callus with shoot and root showed differences.

Keywords


Arachis hypogaea; Immature cotyledon; Regeneration; Seed set; Protein profiles

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