In vitro regeneration from immature cotyledon explant and protein profile changes during organogenesis in groundnut (Arachis hypogaea L.)
Keywords:Arachis hypogaea, Immature cotyledon, Regeneration, Seed set, Protein profiles
AbstractComplete plants were regenerated from in vitro cultured immature cotyledon segments of groundnut (Arachis hypogaea L. cv. TMV-7) by organogenesis. Callus cultures were best initiated from immature cotyledon segments on MS (Murash1ge and Skoog) salts containing B5 vitamins supplemented with indole-3-acetic acid (IAA) and ?-naphthalene acetic acid (NAA; 4.0 mg L·l) and kinetin (KIN; 0.5 mg L ·1). Calluses were transferred to a medium containing KIN (2.0 mg L·1) and IAA and NAA (0. 5 mg L·1) for shoot initiation. The regenerated shoots were transferred to a medium containing indole-3-butyrlc acid (IBA; 2.0 mg L· 1) and KIN (0.2 mg L·1) for developing roots. In vitro produced plantlets developed successfully, matured, and set seed. The protein profiles (sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE)) of callus, callus with shoot, and callus with shoot and root showed differences.