Abstract
Cassava (Manihot esculenta Crantz) cold hardiness by low temperature treatment to plantlets, somatic embryos, and embryonic callus in vitro was studied. The study included the testing of four cassava varieties for cold-hardiness resistant status, cold-temperature injury limit, and the use of electrical conductivity to measure the cold hardiness of different cassava varieties, tissues, and organs. These were used to identify cold-resistant varieties. The effect of different low temperatures and duration of exposure to cold temperature on the cyclic somatic embryogenesis was also studied, and a base was established for cassava somatic cold-resistant mutant selection.
