An improved micropropagation method for the Poyo banana clone is described. Main apices of sucker buds and lateral buds are stimulated by a high concentration of benzyladenine in the absence of auxin. After transplantation, shoot proliferation is achieved with the same concentration of cytokinin. Shoot elongation and rooting are obtained simultaneously on a medium containing no phytohormone and 10 g l-1 sucrose. Rooting is improved when the bottoms of the culture tubes with the medium are set in darkness; rooting is then maximal at 97.9%.