Nonwoven materials as a supporting agent for in vitro culture of banana protocorm-like bodies

Kazumitsu Matsumoto, Hikoyuki Yamaguchi


The use of synthetic nonwoven materials as a support in culture technique to substitute for the agar gelling media in plant tissue culture was studied. Banana protocorm-like bodies (PLBs) were cultured on the nonwoven materials which absorbed a liquid medium. PLBs grew more rapidly on the nonwoven materials than on the agar gelling media. This culture technique should be useful for the in vitro propagation and the selection of mutants.


Banana; Tissue culture; Nonwoven material; Gelling agent; Supporting agent; Protocorm-like body

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