Nonwoven materials as a supporting agent for in vitro culture of banana protocorm-like bodies

Authors

  • Kazumitsu Matsumoto Laboratory of Radiation Genetics and Chemical Mutagenesis, Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, 1-1-1, Yayo1, Bunkyo-ku, Tokyo 113, Japan
  • Hikoyuki Yamaguchi Laboratory of Radiation Genetics and Chemical Mutagenesis, Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, 1-1-1, Yayo1, Bunkyo-ku, Tokyo 113, Japan

Keywords:

Banana, Tissue culture, Nonwoven material, Gelling agent, Supporting agent, Protocorm-like body

Abstract

The use of synthetic nonwoven materials as a support in culture technique to substitute for the agar gelling media in plant tissue culture was studied. Banana protocorm-like bodies (PLBs) were cultured on the nonwoven materials which absorbed a liquid medium. PLBs grew more rapidly on the nonwoven materials than on the agar gelling media. This culture technique should be useful for the in vitro propagation and the selection of mutants.

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Issue

Volume 66 Number 1

Section

Research Papers