Selection of aluminium-tolerant variants from irradiated protocorm-like bodies in banana

Authors

  • Kazumitsu Matsumoto Laboratory of Radiation Genetics and Chemical Mutagenesis, Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan
  • Hikoyuki Yamaguchi Laboratory of Radiation Genetics and Chemical Mutagenesis, Department of Agrobiology, Faculty of Agriculture, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan

Keywords:

Aluminium chloride, Aluminium tolerance, Banana, Musa, Induced mutation, Non-woven material, Protocorm-like body

Abstract

Aluminium-tolerant variants were selected from mutagen-treated protocorm-like bodies (PLBs) of banana cv. Nanicao (Musa cv. AAA group, Cavendish subgroup). PLBs were irradiated by gamma rays for 2kR (0.516 C kg-1) and cultured on a synthetic non-woven material which absorbed the liquid medium containing 10 mM of aluminium chloride. The pH of the medium was adjusted to 4.6 to simulate natural acid soil conditions showing aluminium toxicity for plants. The growing PLBs on the aluminium-containing medium were selected. One of the two PLB lines selected showed a higher tolerance of aluminium stress than the original PLB line. This line was then proliferated and whole plants were obtained.

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Issue

Volume 67 Number 3

Section

Research Papers