Mass production of virus-free patchouli plants [Pogostemon cablin (Blanco) Benth.] by in vitro culture

Authors

  • A.K. Kukreja Central Institute of Medicinal and Aromatic Plants, POB No.1, PO RSM Nagar, Lucknow-226016, India
  • A.K. Mathur Central Institute of Medicinal and Aromatic Plants, POB No.1, PO RSM Nagar, Lucknow-226016, India
  • M. Zaim Central Institute of Medicinal and Aromatic Plants, POB No.1, PO RSM Nagar, Lucknow-226016, India

Keywords:

Meristem culture, Virus elimination, Pogostemon cablin, Patchouli

Abstract

A simple, defined medium has been standardized for the mass multiplication and production of virus-free plants of Pogostemon cablin (Blanco) Benth. Shoot meristems cultured on Murashige and Skoog's (1962) medium (MS) supplemented with 2.0 mg I-1 6-benzylaminopurine (BAP) or 0.5-2.0 mg I-1 6-?,?dimethylallyaminopurine (2iP) or 0.5-2.0 mg I-1 zeatin (Z) and 1.0 mg I-1 indole-3-acetic acid (IAA) exhibited multiple shoot proliferation. Regenerated shoots readily rooted on half-strength MS containing 1.0 mg I-1 IAA or ?-naphthaleneacetic acid (NAA) and 1.5% sucrose. Rooted plantlets were transferred to pots, hardened in the glasshouse and grown to maturity in the field with a high rate (80-90%) of survival. Plant tests made for patchouli mosaic virus (PaMV) with leaf extracts of plants developed in vitro from the fifth subculture passage onwards gave a negative reaction in diagnostic tests on the local lesion host plant, Chenopodium quinoa Willd., demonstrating the absence of the causal agent in plants regenerated from the shoot meristems.

Issue

Section

Research Papers