Effect of media nutrient concentrations on growth of Bradyrhizobium japonicum

Antonio P. Balatti, Luis A. Mazza, M.D. Pastor

Abstract


The attainment in Bradyrhizobium japonicum 5019 cultures of very high cell concentrations was studied by a closed batch system, where all the medium components were incorporated at the beginning of the process. The studies performed allow the establishment of the process conditions that, operating in a stirring fermenter at 400 rev min-1 and employing an aeration rate of 0.5 11-1 min-1 (oxygen absorption rate 513 ml O 2 I-1 h-1), make possible the attainment of a cell concentration of the order of 5 x 1010 viable cells ml-1 in 75 h, using a basic medium containing yeast extract, glycerol and salts.

Keywords


Bradyrhizobium japonicum; Growth; lnoculants; Production

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