The common weed Macroptilium lathyroides is not a source of crop-infecting geminiviruses from Jamaica. (256)

Authors

  • M.E. Roye Biotechnology Centre and Department of Basic Medical Sciences, The University of the West Indies, Mona, Kingston, Jamaica
  • W.A. McLaughlin Biotechnology Centre and Department of Basic Medical Sciences, The University of the West Indies, Mona, Kingston, Jamaica
  • J.D. Spence Biotechnology Centre and Department of Basic Medical Sciences, The University of the West Indies, Mona, Kingston, Jamaica
  • D.P. Maxwell Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison WI 53706, U.S.A.

Keywords:

Macroptilium lathyroides, Geminivirus, Polymerase chain reaction, Jamaica

Abstract

The molecular characterization of bipartite geminiviruses infecting the common leguminous weed Macroptilium lathyroides was accomplished using polymerase chain reaction (PCR), deoxyribonucleic acid (DNA) hybridization, and nucleotide sequence analysis. The predicted PCR-amplified fragments for DNA-A and DNA-B showed that M. lathyroides was infected with bipartite geminiviruses with a genome organization similar to other Western Hemisphere geminiviruses. Nucleotide sequence analysis showed that M. lathyroides from Jamaica was infected with two different viruses. Phylogenetic analysis of these macroptilium viruses from Jamaica and Western Hemisphere viruses, including three viruses from Jamaica indicated that macroptilium golden mosaic virus Jamaica strain 1 (MacGMV-JM1) was phylogenetically related to PYMV and SiGMV-JM, while the other virus, macroptilium golden mosaic virus Jamaica strain 2 (MacGMV-JM2) was closely related to two Jamaican viruses, TDLCV and WGMV. The results suggest that crop and weed geminiviruses from Jamaica are related but distinct, and that M. lathyroides is not host to previously described crop-infecting geminiviruses from Jamaica.

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Issue

Volume 76 Number 4

Section

Research Papers