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Biolistic® transformation of cacao [Theobroma cacao (L.)]. (64)

Research Notes
M.D. Perry
Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
J.B. Power
Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
K.C. Lowe
Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
M.R. Davey
Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
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Keywords

Microprojectile bombardment
Woody plant
Theobroma cacao

How to Cite

Biolistic® transformation of cacao [Theobroma cacao (L.)]. (64). (2000). Tropical Agriculture, 77(1). https://journals.sta.uwi.edu/ojs/index.php/ta/article/view/1441
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Abstract

Callus of cacao [Theobroma cacao (L.)] were transformed by particle bombardment (Biolistics®). Four-week-old floral-derived calli were bombarded with plasmid containing the ?-glucuronidase (gus) gene, driven by a CaMV 35S promoter. Histochemical analysis of gus gene expression was used to optimize the parameters for deoxyribonucleic acid (DNA) delivery. More than 270 histochemically positive (blue staining) transformation events were observed with staminode-derived calli bombarded at 1550 psi.
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