Biolistic® transformation of cacao [Theobroma cacao (L.)]. (64)

Authors

  • M.D. Perry Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
  • J.B. Power Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
  • K.C. Lowe Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.
  • M.R. Davey Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, U.K.

Keywords:

Microprojectile bombardment, Woody plant, Theobroma cacao

Abstract

Callus of cacao [Theobroma cacao (L.)] were transformed by particle bombardment (Biolistics®). Four-week-old floral-derived calli were bombarded with plasmid containing the ?-glucuronidase (gus) gene, driven by a CaMV 35S promoter. Histochemical analysis of gus gene expression was used to optimize the parameters for deoxyribonucleic acid (DNA) delivery. More than 270 histochemically positive (blue staining) transformation events were observed with staminode-derived calli bombarded at 1550 psi.

Issue

Section

Research Notes