, Research Notes

Secondary embryogenesis and plant regeneration in Psidium guajava L. (41)

Research Notes
Jorge A. Vilchez. P.
Dpto. Botánica, Facultad de Agronomía, La Uniuersidad del Zulia, AP 15205, Maracaibo, Edo. Zulia. (4005ZU), Republica Bolivariana de Venezuela
Nilca R. Albany V.
Dpto. Quimica, Facultad de Agronomía, La Universidad del Zulia, AP 15205, Maracaibo, Edo. Zulia. (4005ZU), Republica Bolivariana de Venezuela
Rafael Gomez Kosky
Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas (UCLV), Carretera a Camajuni Km 5 1/2 Santa Clara, Villa Clara, Cuba CP 54830
Leyanis Garcia
Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas (UCLV), Carretera a Camajuni Km 5 1/2 Santa Clara, Villa Clara, Cuba CP 54830
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Keywords

Myrtaceae
Guava
Somatic embryogenesis
In vitro culture
2
4-D

How to Cite

Secondary embryogenesis and plant regeneration in Psidium guajava L. (41). (2004). Tropical Agriculture, 81(1). https://journals.sta.uwi.edu/ojs/index.php/ta/article/view/1223
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Abstract

With the objective of evaluating secondary somatic embryogenesis (SSE) and the germination of somatic embryos of guava (Psidium guajava L.) 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzylaminopurine (6-BAP) were tested, respectively. Fresh weight (FW) and number of secondary somatic embryos formed (NSSEF) in the SSE, and the germination percentage (GP) were determined. The greatest value of NSSEF (48.5; P < 0.05) was obtained with 1 mg L-1 of 2,4-D, eight weeks after culture initiation. The highest GP (83%; P < 0.05) was reached with 0.25 mg L-1 of 6-BAP by culturing the SE in groups. These results suggested that somatic embryogenesis may be a possible alternative for the rapid propagation of promising guava tree selections.
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